BCIP Meaning and
Definition
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BCIP is an acronym that stands for 5-bromo-4-chloro-3-indolyl phosphate, which is a commonly used substrate in various biochemical assays involving alkaline phosphatase. BCIP is a white to off-white crystalline powder that is readily soluble in organic solvents such as dimethylformamide and dimethyl sulfoxide.
In biochemical applications, BCIP is used as a chromogenic substrate to detect and visualize the presence of alkaline phosphatase. It is typically used in conjunction with nitroblue tetrazolium (NBT), which acts as an electron acceptor, resulting in the formation of a purple-colored precipitate. The reaction between BCIP and alkaline phosphatase produces a specific and visible result, indicating the presence and activity of the enzyme in the analyzed sample.
BCIP is widely utilized in various research areas, including immunoblotting, immunocytochemistry, and in situ hybridization. It is particularly effective in identifying and localizing alkaline phosphatase-conjugated antibodies during immunoassays, as well as detecting and visualizing gene expression patterns in tissues. The reaction between BCIP and alkaline phosphatase is highly sensitive, allowing for the accurate detection and quantification of the enzyme's presence.
Overall, BCIP is an essential substrate in biochemical assays that involve alkaline phosphatase, providing researchers with a reliable and convenient method for the detection and visualization of this enzyme in a variety of applications.
Common Misspellings for BCIP
- bciop
- bc9ip
- bci9p
- bc8ip
- bci8p
- bcilp
- bcipl
- bci-p
- bci0p
- bcip0
- bbcip
- bccip
- bciip
- bcipp
- BCyP
- BChP
- b cip
- bc ip
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