FLUORESCENT ANTIBODY TECHNIQUE Meaning and
Definition
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The "Fluorescent Antibody Technique" refers to a laboratory method employed for the detection and localization of specific antigens within biological samples, such as cells or tissues. This technique involves the use of fluorescently labeled antibodies, also known as fluorochromes, that specifically bind to the target antigens present in the sample.
The process begins with the preparation of a sample, followed by the addition of fluorescently labeled antibodies that are directed against a specific antigen of interest. These antibodies typically come from an external source, such as a different species than the sample being analyzed, and are carefully selected to ensure high specificity to the desired antigen.
Once the antibodies are introduced to the sample, they bind to the target antigens, forming a fluorescent antibody-antigen complex. The primary antibodies are typically paired with secondary antibodies that are conjugated with fluorochromes to enhance the signal and allow for visualization.
The sample is then examined under a fluorescence microscope or a similar imaging system that can visualize the fluorescence emitted by the bound antibodies. The fluorescence emitted from the fluorochrome allows for the identification and localization of the specific antigen within the sample.
Overall, the Fluorescent Antibody Technique provides a powerful tool to study the spatial distribution and abundance of specific antigens in biological samples, aiding in the understanding of diverse biological processes and disease mechanisms.
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