ULTRALIGATION Meaning and
Definition
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Ultraligation is a term used primarily in the field of molecular biology to describe a sophisticated technique that involves joining together DNA fragments that have been cleaved or cut using restriction enzymes. It is a highly efficient and precise method used to create recombinant DNA molecules.
During ultraligation, the DNA fragments are treated with specialized enzymes called DNA ligases. These ligases catalyze the formation of phosphodiester bonds between the adjacent ends of the DNA fragments. This enzymatic reaction results in the connection of the DNA fragments and the formation of a continuous, intact DNA molecule.
What distinguishes ultraligation from traditional ligation techniques is the use of modified DNA ligases known as ultraligases. These ultraligases have been engineered through various methods, such as directed evolution, to improve their ligation efficiency and fidelity. They possess enhanced substrate specificity and are capable of joining DNA fragments with higher accuracy and reduced formation of unwanted byproducts.
The application of ultraligation extends beyond molecular cloning and recombinant DNA technology. It has proven to be invaluable in various areas of genetic research and biotechnology, such as DNA sequencing, DNA library construction, and the generation of gene expression constructs. Ultraligation plays a crucial role in these processes, enabling scientists to manipulate DNA molecules with greater precision and reliability.
In summary, ultraligation refers to a specialized DNA ligation technique that utilizes engineered DNA ligases to join DNA fragments, providing researchers with a highly efficient and accurate method for genetic manipulation and engineering.
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Ligation of a bloodvessel beyond the point where a branch is given off.
A practical medical dictionary. By Stedman, Thomas Lathrop. Published 1920.
Common Misspellings for ULTRALIGATION
- yltraligation
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- 7ltraligation
- uktraligation
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- ulrraligation
- ulfraligation
- ulgraligation
- ulyraligation
- ul6raligation
- ul5raligation
- ultealigation
- ultdaligation
- ultfaligation
- ulttaligation
- ult5aligation
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